Nucleotide Sequence and Infectivity of a Full-Length cDNA Clone of Panicum Mosaic Virus

Nucleotide sequence and infectivity of a full-length cDNA clone of panicum mosaic virus.

The sequence of an infectious cDNA clone of panicum mosaic virus (PMV) showed that the single-stranded RNA genome is 4326 nucleotides (nt) and a single highly abundant subgenomic (sg) RNA of 1475 nt was synthesized during PMV infection of pearl millet plants and protoplasts. Computer comparisons revealed strong similarities between the predicted amino acid sequences of the p48 and p112 open rea...

Nucleotide sequence of a full length cDNA clone encoding a polyubiquitin gene from Pisum sativum.

(1989) Nucleotide sequence of a full length cDNA clone encoding a polyubiquitin gene from Pisum sativum. This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published ver...

Sequence Analysis of Expressed cDNA of Bean Common Mosaic Virus RU1 Isolate

Nucleotide sequence of a full length cDNA clone encoding the deoxyribonuclease I from the rat parotid gland.

Monomeric actin interacts with bovine pancreatic DNasel with high affinity (Kb=5xl0 M~') thus forming a stoichiometric 1:1 complex (1). The parotid DNasel has a lower affinity to actin (5.5 x 10 M~') and exhibits no immunologic crossreactivity with the bovine enzyme (2). A parotid specific Xgtll cDNA library has been constructed and screened with a synthetic oligonucleotide sequence deduced fro...

Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion(®) Cloning Kit (...